Plasmenylethanolamine is the major storage depot for arachidonic acid in rabbit vascular smooth muscle and is rapidly hydrolyzed after angiotensin II stimulation

Journal Article · Mon May 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.10.3479· OSTI ID:5300341

Ford, D A; Gross, R W ^[1]

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1. Washington Univ., St. Louis, MO (USA)

The present study demonstrates that rabbit aortic intimal smooth muscle cells contain the majority of their endogenous arachidonic acid mass in plasmenylethanolamine molecular species. To demonstrate the potential significance of these plasmenylethanolamines as substrates for the smooth muscle cell phospholipases that are activated during agonist stimulation, aortic rings were prelabeled with ({sup 3}H)arachidonic acid and stimulated with angiotensin II. Although the specific activities of the choline and inositol glycerophospholipid pools were similar after the labeling interval, ethanolamine glycerophospholipids had a specific activity of only 20% of the specific activity of choline and inositol glycerophospholipids. Despite the marked disparity in the specific activities of these three phospholipid classes, angiotensin II stimulation resulted in similar fractional losses (35-41%) of ({sup 3}H)arachidonic acid from vascular smooth muscle choline, ethanolamine, and inositol glycerophospholipid classes. Reverse-phase HPLC demonstrated that >60% of the ({sup 3}H)arachidonic acid released from ethanolamine glycerophospholipids after angiotensin II stimulation originated from plasmenylethanolamine molecular species. Taken together, the results demonstrate that the major phospholipid storage depot for arachidonic acid in vascular smooth muscle cells are plasmenylethanolamine molecular species which are important substrates for the phospholipase(s) that are activated during agonist stimulation.

• OSTI ID: 5300341
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:10; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANGIOTENSIN
ANIMALS
AORTA
ARACHIDONIC ACID
ARTERIES
BIOCHEMISTRY
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DECOMPOSITION
DRUGS
ENZYMATIC HYDROLYSIS
ESTERS
GLOBULINS
HYDROGEN COMPOUNDS
HYDROLYSIS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LIPIDS
LYSIS
MAMMALS
MASS SPECTROSCOPY
MONOCARBOXYLIC ACIDS
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
RABBITS
SEPARATION PROCESSES
SOLVOLYSIS
SPECTROSCOPY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VERTEBRATES