Serotonin stimulates phospholipase A sub 2 and the release of arachidonic acid in hippocampal neurons by a type 2 serotonin receptor that is independent of inositolphospholipid hydrolysis

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.6.2187· OSTI ID:6073670

Felder, C C; Ma, A L; Axelrod, J; Kanterman, R Y ^[1]

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1. Howard Hughes Medical Institute, Bethesda, MD (USA)

Serotonin (5-HT) stimulated the release of arachidonic acid in hippocampal neurons cocultured with glial cells but not in glial cultures alone. Similar results were observed for the 5-HT-stimulated release of inositol phosphates. These results suggest a neural but not glial origin of both responses. Pharmacological studies suggested that release of arachidonic acid and inositol phosphates was mediated by a type 2 5-HTT (5-HT{sub 2}) receptor. 5-HT-stimulated release of arachidonic acid was also detected in cortical neurons, which contain high levels of 5-HT{sub 2} receptors, but not striatum, spinal cord, or cerebellar granule cells, which have very low levels or are devoid of 5-HT{sub 2} receptors. The phorbol ester phorbol 12-myristate 13-acetate augmented the 5-HT-stimulated release of arachidonic acid but inhibited the 5-HT-stimulated release of inositol phosphates. 5-HT-stimulated release of arachidonic acid, but not inositol phosphates, was dependent on extracellular calcium. 5-HT stimulated the release of ({sup 3}H)lysophosphatidylcholine from ({sup 3}H)choline-labeled cells with no increase in the release of ({sup 3}H)choline or phospho({sup 3}H)choline. These data suggest that 5-HT stimulated the release of arachidonic acid in hippocampal neurons through the activation of phospholipase A{sub 2}, independent of the activation of phospholipase C.

• OSTI ID: 6073670
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:6; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ARACHIDONIC ACID
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BODY
BRAIN
CARBOHYDRATES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
CENTRAL NERVOUS SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYME INDUCTION
ENZYMES
ESTERASES
ESTERS
GENE REGULATION
HETEROCYCLIC COMPOUNDS
HIPPOCAMPUS
HYDROLASES
HYDROXY COMPOUNDS
INDOLES
INOSITOLS
LIPASES
LIPIDS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHARMACOLOGY
PHORBOL ESTERS
PHOSPHOLIPIDS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
SACCHARIDES
SECRETION
SEROTONIN
SYMPATHOMIMETICS
TRYPTAMINES