Muscle protein analysis by two-dimensional gel electrophoresis
Two-dimensional electrophoresis of muscle proteins has provided valuable new information concerning the heterogeneity of some of the major contractile proteins, alterations in the protein population of developing muscle fibers during various stages of myogenesis, and protein aberrations that correlate with muscle diseases. As with all electrophoretic techniques, careful attention must be paid to the preparation of samples and the selection of reagents to be used for the protein separations. Two-dimensional electrophoresis is the obvious method of choice when analysis of protein mixtures is required. The routine clinical application of two-dimensional electrophoresis to analysis of muscle tissue remains to be demonstrated. However, methods of sample preparation for two-dimensional electrophoresis compatible with existing clinical procedures have been described, and the equipment for multiple analyses is available. As protein abnormalities related to human myopathy are detected through the use of two-dimensional electrophoresis as a research tool, useful clinical markers of specific myopathic processes will be found. The preliminary work on muscle protein analysis by two-dimensional electrophoresis described in this review has begun a new approach to the enigma of human muscle disease.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 5299781
- Journal Information:
- CRC Crit. Rev. Clin. Lab. Sci.; (United States), Journal Name: CRC Crit. Rev. Clin. Lab. Sci.; (United States) Vol. 18:1; ISSN CRCLB
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACTIN
BIOLOGICAL VARIABILITY
COLLOIDS
DIAGNOSIS
DISEASES
DISPERSIONS
DOCUMENT TYPES
ELECTROPHORESIS
EVALUATION
GELS
GLOBULINS
MUSCLES
MYOSIN
ONTOGENESIS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
REVIEWS
SAMPLE PREPARATION
TROPOMYOSIN