Muscle protein analysis. I. High-resolution two-dimensional electrophoresis of skeletal muscle proteins for analysis of small biopsy samples

Journal Article · Thu Nov 01 04:00:00 EST 1979 · Clin. Chem.; (United States)

OSTI ID:5368123

Giometti, C S; Anderson, N G; Anderson, N L

A clinically useful method for high-resolution two-dimensional electrophoretic analysis of small (5 to 10 mg) human muscle biopsy samples with sufficient resolution to resolve the major contractile proteins and enzymes is being developed. Using rabbit psoas muscle as a model, we describe methods for sample preparation and two-dimensional electrophoresis. Basic proteins, which appear as streaks when conventional isoelectric focusing is used in the first dimension, are resolved through a modification of the nonequilibrium pH gradient electrophoresis method (Cell 12, 1133 (1977)). In the two-dimensional patterns obtained from rabbit muscle, the components of 10 enzymes and of myosin, actin, tropomyosin, and troponin are identified. These patterns indicate charge heterogeneity in a large fraction of the proteins. Comparison of rabbit and normal human muscle patterns shows many similarities, but much additional work is required to confirm identifications. We conclude that analysis of small biopsy samples is feasible, but that all aspects of human sample acquisition, storage (when necessary), and preparation require thorough study before the method becomes routine in human muscle research and, ultimately, in the diagnosis of some muscle diseases.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5368123
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 25:11; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTIN
BIOPSY
CHEMICAL ANALYSIS
DATA
DATA FORMS
DIAGNOSTIC TECHNIQUES
ELECTROPHORESIS
ENZYMES
EXPERIMENTAL DATA
GLOBULINS
INFORMATION
MUSCLES
MYOSIN
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
SAMPLE PREPARATION