Muscle protein analysis. III. Analysis of solubilized frozen-tissue sections by two-dimensional electrophoresis
Journal Article
·
· Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5631208
Proteins from frozen histological sections of human muscle were analyzed by two-dimensional gel electrophoresis. Patterns so obtained were identical to those from whole homogenates of muscle prepared from frozen tissue powders that had much high protein concentrations. To increase the number of proteins visible on gels of samples low in protein content, the gels were silver stained, or the proteins were labeled with (/sup 14/C)iodoacetamide before electrophoresis and the gels were fluorographed. The latter method allows use of a single frozen-tissue section for two-dimensional electrophoretic analysis and brings the technique closer to practicable clinical use.
- Research Organization:
- Argonne National Lab. Argonne, IL
- OSTI ID:
- 5631208
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:11; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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