Expression of members of immunoglobulin gene family in somatic cell hybrids between human B and T cells
Somatic cell hybrids were obtained between human T and B cells and tested for the expression of differentiated traits of both cell lineages. The T-cell parent SUP-T1 is CD3/sup -/, CD4/sup +/, CD1/sup +/, CD8/sup +/, is weakly positive for HLA class I determinants, and has an inversion of chromosome 14 due to a site-specific recombination event between an immunoglobulin heavy-chain variable gene and the joining segment of the T-cell receptor ..cap alpha.. chain. The B-cell parent, the 6-thioguanine- and ouabain-resistant mutant GM1500, is a lymphoblastoid cell line that secretes IgG2, K chains, and expresses B1, B532, and HLA class I and II antigens. All hybrids expressed characteristics of B cells (Ig/sup +/, B1/sup +/, B532/sup +/, EBNA/sup +/, HLA antigens), whereas only CD4 among the T-cell markers was expressed. The level of T-cell receptor ..beta..-chain transcript was greatly reduced and no RNA of the chimeric T-cell receptor ..cap alpha..-chain joining segment-immunoglobulin heavy-chain variable region was detected. Southern blot analysis indicated that absence of T-cell differentiation markers in the hybrids was not due to chromosomal loss. Rather, some B-cell-specific factor present in the hybrids may account for the suppression.
- Research Organization:
- Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA)
- OSTI ID:
- 5297731
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:14
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNOGLOBULINS
HYBRIDIZATION
LABELLED COMPOUNDS
BIOSYNTHESIS
LYMPHOCYTES
ENZYME IMMUNOASSAY
CELL FLOW SYSTEMS
DNA
GENES
GLUCOPROTEINS
MAN
METHIONINE
PHOSPHORUS 32
SULFUR 35
TUMOR CELLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GLOBULINS
IMMUNOASSAY
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques