Regional distribution and subcellular associations of Type II calcium and calmodulin-dependent protein kinase in rat brain
Four monoclonal antibodies generated against the Type II CaM kinase have been characterized. Two of these antibodies were used to confirm that both alpha and beta subunits were part of the holoenzyme complex. I also developed liquid phase and solid phase radioimmunoassays for the kinase. With the solid phase radioimmunoassay, the distribution of the kinase in rat brain was examined. This study revealed that the concentration of the kinase varies markedly in different brain regions. It is most highly concentrated in the telencephalon where it comprises approximately 2% of total hippocampal protein, 1.3% of cortical protein and 0.7% of striatal protein. It is less concentrated in lower brain regions ranging from 0.3% of hypothalamic protein to 0.1% of protein in the pons/medulla.
- Research Organization:
- California Inst. of Tech., Pasadena (USA)
- OSTI ID:
- 5297369
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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PHOSPHOTRANSFERASES
RADIOIMMUNOASSAY
SUBCELLULAR DISTRIBUTION
BRAIN
MONOCLONAL ANTIBODIES
RATS
ANIMALS
ANTIBODIES
BODY
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
ENZYMES
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IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
NERVOUS SYSTEM
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
TRACER TECHNIQUES
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550201* - Biochemistry- Tracer Techniques