Differential gene expression for glutamic acid decarboxylase and type II calcium-calmodulin-dependent protein kinase in basal ganglia, thalamus, and hypothalamus of the monkey

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Journal of Neuroscience; (United States)

OSTI ID:5509886

Benson, D L; Isackson, P J; Hendry, S H; Jones, E G ^[1]

---

1. Department of Anatomy, University of California, Irvine (USA)

In situ hybridization histochemistry, using cRNA probes, revealed a complementarity in the distributions of cells in the basal ganglia, basal nucleus of Meynert, thalamus, hypothalamus, and rostral part of the midbrain that showed gene expression for glutamic acid decarboxylase (GAD) or the alpha-subunit of type II calcium-calmodulin-dependent protein kinase (CAM II kinase-alpha). Cells in certain nuclei such as the thalamic reticular nucleus, globus pallidus, and pars reticulata of the substantia nigra show GAD gene expression only; others in nuclei such as the basal nucleus of Meynert, medial mamillary nuclei, and ventromedial hypothalamic nuclei show CAM II kinase-alpha gene expression only. A few nuclei, for example, the pars compacta of the substantia nigra and the greater part of the subthalamic nucleus, display gene expression for neither GAD nor CAM II kinase-alpha. In other nuclei, notably those of the dorsal thalamus, and possibly in the striatum, GAD- and CAM II kinase-expressing cells appear to form two separate populations that, in most thalamic nuclei, together account for the total cell population. In situ hybridization reveals large amounts of CAM II kinase-alpha mRNA in the neuropil of most nuclei containing CAM II kinase-alpha-positive cells, suggesting its association with dendritic polyribosomes. The message may thus be translated at those sites, close to the synapses with which the protein is associated. The in situ hybridization results, coupled with those from immunocytochemical staining for CAM II kinase-alpha protein, indicate that CAM II kinase-alpha is commonly found in certain non-GABAergic afferent fiber systems but is not necessarily present in the postsynaptic cells on which they terminate. It appears to be absent from most GABAergic fiber systems but can be present in the cells on which they terminate.

• OSTI ID: 5509886
• Journal Information: Journal of Neuroscience; (United States), Journal Name: Journal of Neuroscience; (United States) Vol. 11:6; ISSN JNRSD; ISSN 0270-6474
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BIOCHEMISTRY
BODY
BRAIN
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DECARBOXYLASES
ENZYMES
GENE REGULATION
GLUTAMIC ACID
HYBRIDIZATION
HYPOTHALAMUS
ISOTOPES
LYASES
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
SULFUR ISOTOPES
THALAMUS
TRANSFERASES