Conformational changes induced by CaS and CaS -calmodulin interaction with brain calmodulin-dependent kinase II are detected with a monoclonal antibody
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6359068
A mouse monoclonal antibody raised against rat brain calmodulin-dependent protein kinase II recognizes the and US subunits of the soluble and cytoskeletal forms of the kinase. Antibody binding was investigated using ( SVI)Bolton-Hunter labeled enzyme. The binding reaction was dependent on CaS in a positively cooperative reaction with an EC50 of 20 M for the cation. The apparent Kd for the antigen itself was determined to be 13 nM by competition with unlabeled ligand. The Mr 30,000 catalytic fragment of the -subunit of the kinase did not bind antibody. Moreover, calmodulin and antibody did not bind to the same site. However, the binding of either calmodulin or antibody to the enzyme potentiated the binding of the other ligand. Antibody-bound enzyme could also be activated more effectively by calmodulin with a marked reduction in Kd and an increase in Vmax. Thus, monoclonal antibodies can serve as useful tools in the study of CaS signalling involving neuronal calmodulin-dependent kinase II.
- Research Organization:
- Wellcome Research Labs., Research Triangle Park, NC
- OSTI ID:
- 6359068
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Monoclonal antibody against brain calmodulin-dependent protein kinase type II detects putative conformational changes induced by Ca/sup 2 +/-calmodulin
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Journal Article
·
Tue Aug 23 00:00:00 EDT 1988
· Biochemistry; (United States)
·
OSTI ID:6470140
Differential recognition of calmodulin-enzyme complexes by a conformation-specific anti-calmodulin monoclonal antibody
Journal Article
·
Tue Nov 04 23:00:00 EST 1986
· J. Biol. Chem.; (United States)
·
OSTI ID:6782097
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Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6366411
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CALCIUM COMPOUNDS
CATIONS
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MONOCLONAL ANTIBODIES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CALCIUM COMPOUNDS
CATIONS
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MONOCLONAL ANTIBODIES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TRANSFERASES