Fate of SV40 DNA in infected cells after treatment with benzo(a)pyrene diol epoxide. [Monkeys]

Journal Article · Tue Jun 16 04:00:00 EDT 1981 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(81)91971-9· OSTI ID:5293395

Chang, G T; Harvey, R G; Weiss, S B

Treatment of SV40-infected monkey kidney cells with antibenzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide results in the alkylation of viral DNA. The specific infectivity of viral DNA isolated from diol epoxide-treated cells is markedly lower than that of viral DNA from untreated cells. Within 5-6 hours following treatment, approximately half of the alkylated viral DNA disappears; during this same period, single-stranded regions in SV40 DNA are detected. These data indicate that, in infected cells, a significant amount of the alkylated viral DNA is removed from the cellular DNA pool while the remainder stays intact. Whether BP-modified SV40 DNA undergoes repair is still uncertain.

• Research Organization: Univ. of Chicago, IL
• DOE Contract Number: AC02-80EV10328
• OSTI ID: 5293395
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 100:3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATION
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CHEMICAL REACTIONS
CONDENSED AROMATICS
DNA
ELECTROPHORESIS
HYDROCARBONS
INHIBITION
KIDNEYS
MAMMALS
MICROORGANISMS
MONKEYS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PRIMATES
REACTION INTERMEDIATES
SIMIAN VIRUS
VERTEBRATES
VIRUSES