Use of short DNA oligonucleotides for determination of DNA sequence modifications induced by benz(a)pyrene diol epoxide

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.81.19.5936· OSTI ID:6134930

Wei, S J.C.; Desai, S M; Harvey, R G; Weiss, S B

In this report, a 10-base-pair oligomer (BamHI linker) is treated with (+/-)-trans-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide and inserted into replicative form DNA of phage M13 by ligation at a specific restriction site. Escherichia coli are transfected with the recombination DNA containing the alkylated target, progeny viral plaques are selected, and their DNAs are subjected to DNA sequence analysis at the region of oligomer insertion. For the alkylated inserts used in this study, the DNA sequence analysis of progeny viral DNA showed that nucleotide deletions were present in every clone examined. These deletions occurred primarily, but not exclusively, at GC cluster regions, varied from 1 to 24 base pairs in length, and included both target and nontarget nucleotides. A second type of repair, which restores most of the original nucleotide bases in the alkylated insert, is also implied by the DNA sequence data obtained. 24 references, 4 figures, 1 table.

• Research Organization: Univ. of Chicago, IL
• DOE Contract Number: AC02-80EV10328
• OSTI ID: 6134930
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 81; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BACTERIOPHAGES
BENZOPYRENE
BIOLOGICAL EFFECTS
CELL CULTURES
CLONE CELLS
CONDENSED AROMATICS
DATA
DNA
DNA SEQUENCING
ESCHERICHIA COLI
EXPERIMENTAL DATA
GENETIC EFFECTS
HYDROCARBONS
INFORMATION
METABOLIC ACTIVATION
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES