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Analysis of the factors which enhance yeast growth and ethanol production

Thesis/Dissertation ·
OSTI ID:5283104
In this study, ethanol fermentation was carried out with a yeast, Saccharomyces cerevisiae. A method for determining the optimum dilution rate in a one-stage continuous stirred tank fermentor (CSTF) was developed to minimize the non-product associated substrate spending. In general, the non-product associated substrate spending could be reduced as dilution rate was increased. Phosphatidylcholine and egg albumin were added as supplements in batch cultures for the purpose of improving ethanol production. The growth rate in batch culture was enhanced by increasing bulk lipid concentration, and depressed by increasing bulk protein concentration. The cell growth in the presence of the lipid-protein complex (supplement) was enhanced by a relatively low level of aeration. Model equations were proposed for cell growth rates and ethanol production rates from which the inhibition constants of glucose and ethanol were estimated for the control and the supplement. The alleviation of the inhibitory effects of glucose and ethanol on cell growth in the supplemented culture as compared to those of the control led to the high cell concentrations which resulted in high fermentor productivities. The increases of specific ethanol productivity in the supplemented culture were also responsible partially for the increases of fermentor productivity.
Research Organization:
Rutgers--the State Univ., New Brunswick, NJ (USA)
OSTI ID:
5283104
Country of Publication:
United States
Language:
English