Production of ethanol by immobilized yeast cells

Journal Article · Sat Aug 01 00:00:00 EDT 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260230809· OSTI ID:5112345

Williams, D; Munnecke, D M

Saccharomyces cerevisiae cells were immobilized in calcium alginate beads for use in the continuous production of ethanol. Yeasts were grown in medium supplemented with ethanol to selectively screen for a culture which showed the greatest tolerance to ethanol inhibition. Yeast beads were produced from a yeast slurry containing 1.5% alginate (w/v) which was added as drops to a 0.05M CaCl2 solution. To determine their optimum fermentation parameters, ethanol production using glucose as a substrate was monitored in batch systems at varying physiological conditions (temperature,pH, ethanol concentration), cell densities, and gel concentrations. The data obtained were compared to optimum free cell ethanol fermentation parameters. The immobilized yeast cells were examined in a packed-bed reactor system operated under optimized parameters derived from batch-immobilized yeast cell experiments. Ethanol production rates, as well as residual sugar concentrations were monitored at different feedstock flow rates. (Refs. 13).

• Research Organization: Dept. Botany-Microbiology, Univ. of Oklahoma, Norman
• OSTI ID: 5112345
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 23:8
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ETHANOL
BIOSYNTHESIS
SACCHAROMYCES CEREVISIAE
CONTINUOUS CULTURE
CULTURE MEDIA
DATA
GLUCOSE
IMMOBILIZED CELLS
PACKED BED
PRODUCTION
YIELDS
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
FUNGI
HEXOSES
HYDROXY COMPOUNDS
INFORMATION
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
SYNTHESIS
YEASTS
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