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Process engineering of high-ethanol-tolerance yeast for the manufacture of ethanol

Conference ·
OSTI ID:150432
; ;  [1]
  1. Purdue Univ., West Lafayette, IN (United States); and others
Inhibitory effects of ethanol and glucose on a high-ethanol-tolerance yeast strain (fusion product of Saccharomyces diastaticus and Saccharomyces uvarum) having high osmotic and ethanol tolerance were studied in batch cultures. A model incorporating both substrate and product inhibition was developed that represented the experimental data quite well. By performing fed-batch fermentation, an ethanol concentration of 13.3% (w/v) was obtained. The maximum allowable ethanol concentration for cell growth was predicted to be 129.9 g/L and ethanol-producing capability of cells was found to be completely inhibited at 136.4 g/L. On-line monitoring of the fermentation was performed using an ion trap mass spectrometer and a triple quadrupole mass spectrometer. Preliminary results are reported.
Research Organization:
Oak Ridge National Lab., TN (United States)
OSTI ID:
150432
Report Number(s):
CONF-940526--
Country of Publication:
United States
Language:
English

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