General method for the isolation and analysis of polynucleotide fragments cross-linked to protein
Journal Article
·
· Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)
OSTI ID:5280799
A general method is proposed for the isolation and sequencing of fragments of polynucleotides cross-linked to a definite protein among multicomponent nucleoproteins. The method can be used for initially unlabeled complexes regardless of the method of forming the cross-linkages. With the aid of this method, a fragment of the 16S RNA covalently cross-linked to the S7 protein under the action of ultraviolet radiation (254 nm) on the 30S subunit of E. coli ribosomes has been isolated. The primary structure of this fragment has been determined (CUACAAUG) and it has been established that the S7 protein is covalently bound (cross-linked) on the irradiation of the free 30S subunit with the U/sup 1239/ residue of the 16S RNA, which agrees with results obtained previously.
- Research Organization:
- M. M. Shemyakin Institute of Bioorganic Chemistry, Moscow (USSR)
- OSTI ID:
- 5280799
- Journal Information:
- Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Journal Name: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States) Vol. 11:10; ISSN SJBCD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
COMPLEXOMETRY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENETIC MAPPING
HYDROLASES
HYDROLYSIS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSIS
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDASES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PHOTOCHEMICAL REACTIONS
POLYMERIZATION
PROTEINS
RADIATIONS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RNA
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
ULTRAVIOLET RADIATION
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
COMPLEXOMETRY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENETIC MAPPING
HYDROLASES
HYDROLYSIS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSIS
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDASES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PHOTOCHEMICAL REACTIONS
POLYMERIZATION
PROTEINS
RADIATIONS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RNA
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
ULTRAVIOLET RADIATION