Cross-linking of streptomycin to the 16S ribosomal RNA of Escherichia coli

Journal Article · Tue Sep 22 00:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00393a041· OSTI ID:5640003

Gravel, M; Melancon, P; Barkier-Gingras, L

(/sup 3/H)Dihydrostreptomycin was cross-linked to the 30S ribosomal subunit from Escherichia coli with the bifunctional reagent nitrogen mustard. The cross-linking primarily involved the 16S RNA. To localize the site of cross-linking of streptomycin to the 16S RNA, the authors hybridized RNA labeled with streptomycin to restriction fragments of the 16S RNA gene. Labeled RNA hybridized to DNA fragments corresponding to bases 892-917 and bases 1394-1415. These two segments of the ribosomal RNA must by juxtaposed in the ribosome, since there is a single binding site for streptomycin. This region has been implicated both in the decoding site and in the binding of initiation factor IF-3, indicating its functional importance.

• Research Organization: Universite de Montreal, Quebec
• OSTI ID: 5640003
• Journal Information: Biochemistry; (United States), Vol. 26:19
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RIBOSOMAL RNA
CROSS-LINKING
HYBRIDIZATION
STREPTOMYCIN
TRITIUM COMPOUNDS
ESCHERICHIA COLI
GENES
PHOSPHORUS 32
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
POLYMERIZATION
RADIOISOTOPES
RNA
550201* - Biochemistry- Tracer Techniques