Evidence for photoinduced cross-linkage, in situ, of 30S ribosomal proteins to 16S rRNA

Journal Article · Tue Oct 21 04:00:00 EDT 1975 · Biochemistry, v. 14, no. 21, pp. 4627-4633

OSTI ID:4153821

Gorelic, L

The effects of ultraviolet radiation on the 30S ribosomal subunit of Escherichia coli were studied. Irradiation in aqueous solution under anaerobic conditions resulted in a dose-dependent decrease in the separability of the rRNA and protein components of the 30S ribosomal sub-unit in 4 M urea-3 M LiCl. The results of gel filtration studies of the irradiated ribosomes beforeand after treatment with pancreatic ribonuclease indicated that the decrease in separability of the ribosome components was a result of the photoinduced formation of covalent RNA-protein cross-links. The number of covalent cross- links estimated to correspond to less than 3 per 10,00 daltons of ribosomal proteins. One-dimensional gel electrophoresis studies of the course of the photoinduced cross-linkage reaction indicated that cross-linkage of individual 30S ribosomal proteins to the 16S rRNA proceeds in two dose-dependent steps. The first step requires an input of 1 x 10$sup 20$ quanta of 253.7-nm radiation and results in the cross-linkage of at least five ribosomal proteins to the 16S rRNA. The second step requires a total input of 2 x 10$sup 20$ quanta of 253.7-nm radiation, and results in the cross-linkage of most of the remaining 30S ribosomal proteins to the 16S rRNA. (auth)

• Research Organization: Wayne State Univ., Detroit
• NSA Number: NSA-33-003160
• OSTI ID: 4153821
• Journal Information: Biochemistry, v. 14, no. 21, pp. 4627-4633, Journal Name: Biochemistry, v. 14, no. 21, pp. 4627-4633; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

*PROTEINS-- RIBOSOMES
*RIBOSOMES-- BIOLOGICAL RADIATION EFFECTS
*RNA-- CROSS-LINKING
*ULTRAVIOLET RADIATION-- BIOLOGICAL RADIATION EFFECTS
560111* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Biochemicals--In Vitro
AQUEOUS SOLUTIONS
DOSE-RESPONSE RELATIONSHIPS
ELECTROPHORESIS
ESCHERICHIA COLI
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
PANCREAS
RADIOINDUCTION
RNA-ASE
UREA