Method to transform algae, materials therefor, and products produced thereby

Patent · Tue Aug 26 04:00:00 EDT 1997

OSTI ID:527757

Dunahay, T G; Roessler, P G; Jarvis, E E

Disclosed is a method to transform chlorophyll C-containing algae. The method includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further, specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae. 2 figs.

• Research Organization: Midwest Research Institute
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC36-83CH10093
• Assignee: PTO; PA: EDB-97:127301; SN: 97001843779; SCA: 550200
• Patent Number(s): US 5,661,017/A/
• Application Number: PAN: 8-404,732
• OSTI ID: 527757
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ALGAE
CHLOROPHYLL
GENETIC ENGINEERING
NUCLEIC ACIDS
RECOMBINANT DNA