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Chimeric enzymes with improved cellulase activities

Patent ·
OSTI ID:1177097
; ;
Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.
Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,993,276
Application Number:
13/940,936
OSTI ID:
1177097
Country of Publication:
United States
Language:
English

References (11)

Construction and characterization of chimeric cellulases with enhanced catalytic activity towards insoluble cellulosic substrates journal May 2012
Cellulases and biofuels journal June 2009
Optimizing the stability of single-chain proteins by linker length and composition mutagenesis journal May 1998
Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725 journal January 2011
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Multidomain and Multifunctional Glycosyl Hydrolases from the Extreme Thermophile Caldicellulosiruptor Isolate Tok7B.1 journal May 2000
Cloning of Thermostable Cellulase Genes of Clostridium thermocellum and Their Secretive Expression in Bacillus subtilis journal November 2011
CelI, a Noncellulosomal Family 9 Enzyme from Clostridium thermocellum, Is a Processive Endoglucanase That Degrades Crystalline Cellulose journal January 2003
Glycoside hydrolase inventory drives plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus journal March 2011
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Protein Disorder: Conformational Distribution of the Flexible Linker in a Chimeric Double Cellulase journal April 2005

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES