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Enhanced processive cellulases

Patent ·
OSTI ID:1364629
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Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.
Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
Assignee:
Alliance for Sustainable Energy, LLC
Patent Number(s):
9,683,248
Application Number:
14/365,200
OSTI ID:
1364629
Country of Publication:
United States
Language:
English

References (6)

Cellulases and biofuels journal June 2009
SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability journal July 2009
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei journal January 1987
A family of thermostable fungal cellulases created by structure-guided recombination journal March 2009
Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose journal September 1997
Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source journal July 2010

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59 BASIC BIOLOGICAL SCIENCES