Enhanced processive cellulases

Adney, William S.; Beckham, Gregg T.; Jarvis, Eric; Himmel, Michael E.; Decker, Stephen R.; Linger, Jeffrey G.; Podkaminer, Kara; Baker, John O.; Taylor, II, Larry; Xu, Qi; Singh, Arjun

Enhanced processive cellulases

Patent · Tue Jul 31 04:00:00 EDT 2018

OSTI ID:1464739

Adney, William S.; Beckham, Gregg T.; Jarvis, Eric; Himmel, Michael E.; Decker, Stephen R.; Linger, Jeffrey G.; Podkaminer, Kara; Baker, John O.; Taylor, II, Larry; Xu, Qi; Singh, Arjun

Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC36-08GO28308

Assignee:: Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Number(s):: 10,036,051

Application Number:: 15/594,031

OSTI ID:: 1464739

Country of Publication:: United States

Language:: English

References (11)

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Role of the interdomain linker peptide of Trichoderma reesei cellobiohydrolase I in its interaction with crystalline cellulose. Srisodsuk, M.; Reinikainen, T.; Penttilä, M. Journal of Biological Chemistry, Vol. 268, Issue 28 https://doi.org/10.1016/S0021-9258(19)36847-4	journal	October 1993
Developments in Directed Evolution for Improving Enzyme Functions Sen, S.; Venkata Dasu, V.; Mandal, B. Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223 https://doi.org/10.1007/s12010-007-8003-4	journal	August 2007
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Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose Srisodsuk, Malee; Lehtiö, Janne; Linder, Markus Journal of Biotechnology, Vol. 57, Issue 1-3, p. 49-57 https://doi.org/10.1016/S0168-1656(97)00088-6	journal	September 1997
Probing the role of N-linked glycans in the stability and activity of fungal cellobiohydrolases by mutational analysis Adney, William S.; Jeoh, Tina; Beckham, Gregg T. Cellulose, Vol. 16, Issue 4 https://doi.org/10.1007/s10570-009-9305-1	journal	June 2009
Binding modules alter the activity of chimeric cellulases: Effects of biomass pretreatment and enzyme source Kim, Tae-Wan; Chokhawala, Harshal A.; Nadler, Dana Biotechnology and Bioengineering, Vol. 107, Issue 4, p. 601-611 https://doi.org/10.1002/bit.22856	journal	July 2010
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