Method to transform algae, materials therefor, and products produced thereby

Dunahay, Terri Goodman; Roessler, Paul G; Jarvis, Eric E

Method to transform algae, materials therefor, and products produced thereby

Patent · Wed Jan 01 04:00:00 EST 1997

OSTI ID:871113

Dunahay, Terri Goodman ^[1]; Roessler, Paul G ^[2]; Jarvis, Eric E ^[3]

2710 Arbor Glen Pl., Boulder, CO 80304
15905 Ellsworth Pl., Golden, CO 80401
3720 Smuggler Pl., Boulder, CO 80303

Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.

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Research Organization:: Midwest Research Institute

DOE Contract Number:: AC36-83CH10093

Assignee:: Dunahay, Terri Goodman (2710 Arbor Glen Pl., Boulder, CO 80304);Roessler, Paul G. (15905 Ellsworth Pl., Golden, CO 80401);Jarvis, Eric E. (3720 Smuggler Pl., Boulder, CO 80303)

Patent Number(s):: US 5661017

Application Number:: 08/404732

OSTI ID:: 871113

Country of Publication:: United States

Language:: English

References (19)

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/435/999/
ability
accomplish
acid
acid molecule
alga
algae
algal
c-containing
chimeric
chlorophyll
comprising
control
control sequence
control sequences
cryptica
cyclotella
cyclotella cryptica
derived
diatom
disclosed
dominant
embodiment
encoding
genetic
introducing
linked
manner
marker
materials
method
methods
molecule
molecule comprising
molecules
normally
normally occur
nucleic
nucleic acid
occur
operatively
operatively linked
paccnpt10
paccnpt4
paccnpt5
preferably
preferred
preferred embodiment
produced
products
products produced
protein
provide
recombinant
recombinant molecule
regulatory
rna
selectable
sequence
sequences
specifically
specifically disclosed
stable
transform
transformation

Method to transform algae, materials therefor, and products produced thereby

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References (19)

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