Differential distribution and placental transport of 2- and 3-t-(methyl-14C)butyl-4-hydroxyanisole (BHA) in pregnant mice

Journal Article · Thu Jan 31 23:00:00 EST 1991 · Fundamental and Applied Toxicology; (United States)

DOI:https://doi.org/10.1016/0272-0590(91)90120-S· OSTI ID:5270434

Ahmed, A E; Ansari, G A; Dencker, L; Ullberg, S ^[1]

---

1. Univ. of Texas Medical Branch, Galveston (USA)

The placental transport and localization in fetal and maternal tissues of 14C-BHA isomers, 2-t-(methyl-14C)butyl-4-hydroxyanisole (2-BHA) and 3-t-(methyl-14C)butyl-4-hydroxyanisole (3-BHA), were studied in pregnant mice by whole-body autoradiography techniques. BHA isomers were given (iv 50 microCi/100 g as a tracer dose) to pregnant mice at Day 11 (organogenesis) and Day 18 (postorganogenesis) of gestation. Peak levels of radioactivity occurred in various tissues 1-4 hr after iv administration of both isomers. 3-BHA and its metabolites have a higher affinity to fatty tissues and livers of pregnant mice. The concentration of radiocarbon in maternal liver and brown fat following treatment with 14C-3-BHA was much higher than the radioactivity concentration in the corresponding tissues of mothers treated with 2-BHA. On the other hand, the fetal concentration of radioactivity was higher in animals treated with 2-BHA than in those treated with 3-BHA. The radioactivity derived from both isomers accumulated in the fetal gastrointestinal tract. In both groups the radioactivity accumulated in the maternal nasal cavity and mucosa and the gastrointestinal contents. At 24 hr after treatment, retention of radioactivity in maternal lungs, amniotic fluid, and fetal gastrointestinal tissues was observed. Results from this study indicate that there are differences in the magnitude and extent of placental transport of 3-BHA and 2-BHA. Differences also exist in maternal organ uptake and radioactivity distribution of both isomers. Findings from this study are consistent with pharmacological differences existing between the isomers.

• OSTI ID: 5270434
• Journal Information: Fundamental and Applied Toxicology; (United States), Journal Name: Fundamental and Applied Toxicology; (United States) Vol. 16:2; ISSN FAATD; ISSN 0272-0590
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANISOLE
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
DISTRIBUTION
ETHERS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PREGNANCY
RODENTS
TISSUE DISTRIBUTION
VERTEBRATES