Isolation and determination of benzo(a)pyrene glucuronide and sulfate conjugates in soybean leaves

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Bull. Environ. Contam. Toxicol.; (United States)

DOI:https://doi.org/10.1007/BF01689420· OSTI ID:5269623

Negishi, T; Nakano, M; Kobayashi, S; Kim, C H

BaP is metabolized in mammalian systems by the mixed function oxidase system of liver microsomes. This system catalyzes the oxidation of BaP via epoxide intermediate to phenol, diol and quinone metabolites. One of these 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydro-BaP is thought to act as the ultimate carcinogen by binding covalently to cellular DNA. It is also known that Cunninghamella elegans oxidized BaP to its phenol, diol and quinone metabolites. In addition, the alcohols were detected as glucuronide and sulfate conjugates. These metabolites are remarkably similar to those observed in higher organisms. On the other hand, some investigators have demonstrated that plants take up BaP and anthracene from soil or culture medium containing these compounds. This paper reports the finding that soybeans grown in BaP polluted soil take it up and metabolize to its phenol, diol and the glucuronide and sulfate conjugates of the alcohols.

• Research Organization: Obihiro Univ. of Agriculture and Veterinary Medicine (Japan)
• OSTI ID: 5269623
• Journal Information: Bull. Environ. Contam. Toxicol.; (United States), Journal Name: Bull. Environ. Contam. Toxicol.; (United States) Vol. 39:2; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AROMATICS
BENZOPYRENE
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
FLUORESCENCE
FOOD
GLUCURONIC ACID
GROWTH
HYDROCARBONS
HYDROXY ACIDS
LEAVES
LUMINESCENCE
MASS SPECTRA
METABOLITES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANT GROWTH
PLANTS
SEPARATION PROCESSES
SOILS
SOYBEANS
SPECTRA
SULFATES
SULFUR COMPOUNDS
THIN-LAYER CHROMATOGRAPHY
UPTAKE
VEGETABLES