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Oxidation of benzo(a)pyrene by the filamentous fungus Cunninghamella elegans

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:5340073
;

Cunninghamella elegans oxidized benzo(a)pyrene to several metabolic products. Compounds that were isolated and identified were: trans-9,10-dihydroxy-9,10-dihydrobenzo(a)pyrene, trans-7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene, benzo(a)pyrene 1,6-quinone, benzo(a)pyrene 3,6-quinone, 9-hydroxybenzo(a)pyrene, and 3-hydroxybenzo(a)pyrene. In addition, an unidentified dihydroxybenzo(a)pyrene metabolite was also formed. Experiments with (/sup 14/C)benzo(a)pyrene showed that over a 96-h period, 18.4% of the hydrocarbon was converted to metabolic products. Most of the metabolites were sulfate conjugates as demonstrated by the formation of benzo(a)pyrene quinones and phenols after treatment with aryl sulfatase. Glucuronide and sulfate conjugates were also detected as water-soluble metabolites. The results show that benzo(a)pyrene is metabolized by a filamentous fungus in a manner that is remarkably similar to that observed in higher organisms.

Research Organization:
Univ. of Texas, Austin
OSTI ID:
5340073
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:23; ISSN JBCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550701 -- Microbiology-- Tracer Techniques
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBON 14
CARBON ISOTOPES
CHEMICAL REACTIONS
CONDENSED AROMATICS
EVEN-EVEN NUCLEI
FUNGI
HYDROCARBONS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
METABOLISM
METABOLITES
NUCLEI
ORGANIC COMPOUNDS
OXIDATION
PLANTS
RADIOISOTOPES
REACTION KINETICS
TOLERANCE
TOXICITY
YEARS LIVING RADIOISOTOPES