Rat bladder cell-mediated mutagenesis of Chinese hamster V79 cells and metabolism of benzo(a)pyrene

Journal Article · Fri May 01 04:00:00 EDT 1981 · J. Natl. Cancer Inst.; (United States)

OSTI ID:6363126

Langenbach, R ^[1]; Malick, L; Nesnow, S

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1. Environmental Protection Agency, Research Triangle Park, MD

Primary rat bladder epithelial cells were cocultivated with Chinese hamster V79 cells in the presence of carcinogens, and the induction of 6-thioguanine resistance in the V79 cells was used as a market of cell-mediated mutagenesis. The carcinogens dimethylnitrosamine, 7,12-dimethylbenz(a)anthracene, and benzo(a)pyrene (BP) were mutagenic to V79 cells in the presence of bladder cells but not in their absence. Analysis of BP metabolites (formed by bladder cells indicated that 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene, 9,10-dihydro-9,10-dihydroxybenzo(a)pyrene, benzo(a)pyrene-3,6-quinone, and 9-hydroxybenzo(a)pyrene were the major organic-soluble metabolites formed. The finding that rat bladder peithelium can metabolize some carcinogens offers new possibilities for the mechanism of initiation of bladder cancer.

• OSTI ID: 6363126
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 66:5; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BLADDER
BODY
CELL CULTURES
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DIMETHYLBENZANTHRACENE
FIBROBLASTS
HAMSTERS
HYDROCARBONS
MAMMALS
METABOLISM
MUTAGENESIS
NITROSO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RATS
RODENTS
SOMATIC CELLS
URINARY TRACT
VERTEBRATES