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Two distinct pools of membrane phosphatidylglycerol in Bacillus megaterium

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:5263959
;
The predominant membrane lipid in Bacillus megaterium ATCC 14581, phosphatidylglycerol (PG), is present in two distinct pools, as shown by (/sup 32/P)phosphate incorporation and chase experiments. One pool (PG/sub t/) undergoes rapid turnover of the phosphate moiety, whereas the second pool (PG/sub s/) exhibits metabolic stability in this group. The phosphate moiety of the other major phospholipid, phosphatidylethanolamine, is stable to turnover. (/sup 32/P)phosphate- and (2-/sup 3/H)glycerol-equilibrated cultures yielded the following glycerolipid composition: 56 mol% PG (34 mol% PG/sub t/ and 22 mol% PG/sub s/), 21 mol% phosphatidylethanolamine, 1 to 2 mol% phosphatidylserine, 20 mol% diglycerides, less than 0.5 mol% cardiolipin, and 0.2 to 0.4 mol% lysophosphatidylglycerol. Accumulation of PG was halted immediately after the addition of cerulenin, an inhibitor of de novo fatty acid synthesis, whereas phosphatidylethanolamine accumulation continued at the expense of the diglyceride and PG pools. Strikingly, initial rates of (/sup 32/P)phosphate incorporation into PG were unaffected by cerulenin. In control cultures at 35/sup 0/C, incorporation of (/sup 32/P)phosphate into PG exhibited a biphasic time course, whereas incorporation into phosphatidylethanolamine was concave upward and lagged behind that of PG during the initial rapid phase of PG incorporation. Finally, levels of lysophosphatidylglycerolexpanded rapidly after cerulenin addition at 20/sup 0/C, but not at 35/sup 0/C. Moreover, incorporation of (/sup 32/P)phosphate into lysophosphatidylglycerol lagged behind incorporation into PG in both the presence and absence of cerulenin at 20 and 35/sup 0/C.
Research Organization:
Univ. of California Medical School, Los Angeles
OSTI ID:
5263959
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 141:2; ISSN JOBAA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BACILLUS
BACILLUS MEGATERIUM
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CARDIOLIPIN
CELL CULTURES
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ESTERS
EXPERIMENTAL DATA
GLYCEROL
GRAPHS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INFORMATION
INHIBITION
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MEMBRANES
METABOLISM
MICROORGANISMS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORIC ACID ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
TRITIUM
YEARS LIVING RADIOISOTOPES