Turnover and metabolism of phosphatidylglycerol acyl moieties in E. coli

Conference · Fri May 01 04:00:00 EDT 1987 · Federation Proceedings, Federation of American Societies for Experimental Biology; (USA)

OSTI ID:5051675

Cooper, C L; Rock, C O ^[1]

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1. St. Jude Children's Research Hospital, Memphis, TN (USA)

Fatty acids synthesized in mutants (plsB) blocked in de novo phospholipid biosynthesis were preferentially transferred to phosphatidylglycerol (PtdGro). The ratio of phospholipid species labeled with {sup 32}P and ({sup 3}H)acetate in the absence of glycerol-3-P acyltransferase activity indicated that ({sup 3}H)acetate incorporation into PtdGro was due to fatty acid turnover. The magnitude of the turnover process was difficult to estimate due to a significant contraction of the acetyl-CoA pool following the inhibition of phospholipid synthesis. A possible connection between PtdGro turnover and protein acylation was investigated in an E. coli strain containing a lipoprotein expression vector. Cells were prelabeled with ({sup 3}H)acetate and lipoprotein expression was induced concomitant with the addition of exogenous ({sup 14}C)-palmitate. ({sup 14}C) Palmitate was assimilated into the l-position of phosphatidylethanolamine and transferred to the amino terminus of the lipoprotein. In contrast, the ester-linked lipoprotein fatty acids and PtdGro were not enriched in carbon-14 implying a metabolic relationship between these two pools. The data suggest that turnover of PtdGro acyl moieties is related to protein acylation, but a direct link between the two processes remains to be established.

• OSTI ID: 5051675
• Report Number(s): CONF-870644--
• Conference Information: Journal Name: Federation Proceedings, Federation of American Societies for Experimental Biology; (USA) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID ESTERS
ACYLATION
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COENZYMES
DAYS LIVING RADIOISOTOPES
ENZYMES
ESCHERICHIA COLI
ESTERS
GLYCOLIPIDS
HEXADECANOIC ACID
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MUTANTS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS