Biosynthesis of unsaturated fatty acides by bacilli. Hyperinduction and modulation of desaturase synthesis. [Bacillus megaterium]
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:5373348
Significant relationships have been established between the initiation and rate of fatty acid ..delta../sup 5/-desaturase synthesis in Bacillus megaterium ATCC 14581 and controlled perturbations in culture temperature, cell growth, protein synthesis, and RNA synthesis. B. megaterium growing from inoculum at 35/sup 0/ contained neither unsaturated fatty acids nor the ..delta../sup 5/-desaturase responsible for their production. When the culture temperature was lowered rapidly to 20/sup 0/, synthesis of desaturase began within 5 min, attained a maximum rate at about 15 min, and continued at this high rate for up to 90 min after the shift to 20/sup 0/. This ''hyperinduction'' process, so-called because the rate of desaturase synthesis after culture transfer from 35 to 20/sup 0/ far exceeded the rate found in comparable cultures growing from inoculum at 20/sup 0/, was dependent on protein synthesis and RNA synthesis initiated after transfer. Rifampicin, added at the time of culture transfer, completely blocked the appearance of desaturase activity. The maximum rate of desaturase synthesis achieved during hyperinduction, when normalized for the concurrent rate of protein synthesis, was always a constant, regardless of experimental conditions. The hyperinduction phase was followed by a period of rapid attenuation of desaturase synthesis until the rate was at or below that in comparable cultures grown at 20/sup 0/ from inoculum.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 5373348
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 252:11; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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