Radioimmunoassay analysis of baculovirus granulins and polyhedrins
Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera. Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.
- Research Organization:
- Texas A and M Univ., College Station
- OSTI ID:
- 5246232
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 103:1; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL PREPARATION
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
ELECTRON CAPTURE RADIOISOTOPES
ENCAPSULATION
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
VIRUSES