Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Journal Article · Thu May 01 00:00:00 EDT 1980 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(80)90128-2· OSTI ID:5246232

Summers, M D; Hoops, P

Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera. Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.

• Research Organization: Texas A and M Univ., College Station
• OSTI ID: 5246232
• Journal Information: Virology; (United States), Vol. 103:1
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
NUCLEOPROTEINS
RADIOIMMUNOASSAY
BIOCHEMICAL REACTION KINETICS
CHEMICAL PREPARATION
DIAGNOSIS
ENCAPSULATION
IMMUNE SERUMS
IODINE 125
VIRUSES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
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NUCLEI
ODD-EVEN NUCLEI
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PARASITES
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RADIOASSAY
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550701 - Microbiology- Tracer Techniques