Production of human beta interferon in insect cells infected with a Baculovirus expression vector

Journal Article · Wed Nov 30 23:00:00 EST 1983 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.3.12.2156· OSTI ID:5943392

Smith, G E; Summers, M D; Fraser, M J

Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences for interferon were linked to the AcNPV promoter for the gene encoding for polyhedrin, the major occlusion protein. The interferon gene was inserted at various locations relative to the AcNPV polyhedrin transcriptional and translational signals, and the interferon-polyhedrin hybrid genes were transferred to infectious AcNPV expression vectors. Biologically active interferon was produced, and greater than 95% was secreted from infected insect cells. A maximum of ca. 5 x 10/sup 6/ U of interferon activity was produced by 10/sup 6/ infected cells. These results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes.

• Research Organization: Dept. of Entomology, Texas A and M Univ., and Texas Agricultural Experiment Station, College Station, TX 77843
• OSTI ID: 5943392
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 3:12; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTHROPODS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BIOTECHNOLOGY
CELL CONSTITUENTS
CELL CULTURES
CLASSIFICATION
CONTROL
ENZYME INHIBITORS
FUNCTIONS
GENES
GENETIC CONTROL
GENETIC ENGINEERING
GROWTH FACTORS
HUMAN POPULATIONS
HYBRIDIZATION
INSECTS
INTERFERON
INVERTEBRATES
LYMPHOKINES
MICROORGANISMS
MITOGENS
ORGANIC COMPOUNDS
PARASITES
PEST CONTROL
PLASMIDS
POPULATIONS
PROTEINS
SYNTHESIS
TRANSCRIPTION
VIRUSES