Expression of the glycoprotein gene from a fish rhabdovirus by using baculovirus vectors
Journal Article
·
· Journal of Virology; (United States)
OSTI ID:7261061
- Oregon State Univ., Corvallis (United States)
A cDNA fragment containing the gene encoding the glycoprotein of infectious hematopoietic necrosis virus was inserted into Autographa californica baculovirus vectors under the control of the polyhedrin promoter. A 66-kilodalton protein, identical in size to the glycosylated glycoprotein of infectious hematopoietic necrosis virus, was expressed at high levels in Spodoptera frugiperda cells infected with the recombinant viruses. The expressed protein reacted with antiserum to the glycoprotein on Western blots.
- OSTI ID:
- 7261061
- Journal Information:
- Journal of Virology; (United States), Journal Name: Journal of Virology; (United States) Vol. 64:1; ISSN 0022-538X; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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