Decreased (/sup 3/H)nitrendipine binding in the brainstem of deoxycorticosterone-NaCl hypertensive rats

Journal Article · Mon Sep 09 04:00:00 EDT 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90534-X· OSTI ID:5244199

Lee, H R; Watson, M; Yamamura, H I; Roeske, W R

Binding studies with the 1,4 dihydrophyridine calcium channel antagonist (/sup 3/H)nitrendipine ((/sup 3/H)NTD) were performed in uninephrectomized, deoxycorticosterone (DOCA)-NaCl hypertensive rats and vehicle treated normotensive control littermates. After 6 weeks of treatment, hypertensive (199 mmHg, systolic arterial pressure) DOCA rats showed significantly increased heart, left ventricle, and kidney weight in contrast to normotensive (135 mmHg) controls. (/sup 3/H)NTD binding in the brainstem was significantly reduced (51 +/- 5 fmol/mg protein) in DOCA-NaCl rats, as compared to controls (116 +/- 24 fmol/mg protein). However, no significant differences were found in the (/sup 3/H)NTD dissociation constants for DOCA-NaCl (0.43 +/- 0.03 nM) or control rats (0.62 +/- 0.06 nM). Cerebral cortical and left ventricular tissue showed no significant alterations in receptor binding density or affinity. Specific (/sup 3/H)NTD binding was not significantly altered in other selected brain regions or the atria. These data suggest that alterations in the dihydropyridine binding sites associated with calcium channels in the brainstem may be involved in the etiology of DOCA-NaCl-induced hypertension. 34 references, 1 figure, 2 tables.

• Research Organization: Univ. of Arizona Health Science Center, Tucson
• OSTI ID: 5244199
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:10; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALKALINE EARTH METALS
ANIMALS
AZINES
BLOOD PRESSURE
BODY
BRAIN
CALCIUM
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CHEMICAL BONDS
DATA
DISEASES
ELEMENTS
ETIOLOGY
EXPERIMENTAL DATA
HEART
HETEROCYCLIC COMPOUNDS
HYPERTENSION
INFORMATION
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
METALS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIDINES
RATS
RECEPTORS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASCULAR DISEASES
VERTEBRATES
WEIGHT