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Synaptosomal calcium influx is activated by sodium fluoride

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
;
Neuronal calcium channels can be modulated by changes in membrane potential or by activation of channel-associated receptors. The latter may be modulated by guanine nucleotide binding proteins. NaF, which activates guanine nucleotide binding proteins, caused a large stimulation of /sup 45/Ca/sup 2 +/ uptake by synaptosomes prepared from rat brain. Stimulation of /sup 45/Ca/sup 2 +/ influx by NaF (i) was apparent in media containing either 5 mM-K+ or 50 mM-K+, (ii) was slower than the fast-phase of voltage-dependent /sup 45/Ca/sup 2 +/ influx but continued for a longer period of time than did depolarization-induced /sup 45/Ca/sup 2 +/ influx, and (iii) was not mimicked or modified by a number of drugs, including ouabain, dinitrophenol, sodium azide or sodium vanadate. These results are consistent with the hypothesis that NaF activates a guanine nucleotide binding protein associated with receptor-coupled calcium channels, resulting in stimulation of calcium influx.
Research Organization:
Univ. of Alabama, Birmingham (USA)
OSTI ID:
5244020
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 151:2; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METALS
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
ORGANIC COMPOUNDS
ORGANS
POTASSIUM
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RESPONSE MODIFYING FACTORS
RODENTS
SODIUM COMPOUNDS
SODIUM FLUORIDES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES