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Amino acid receptors mediate calcium permeability in synaptosomes from rat brain

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5019728
;
Selected acidic amino acids and amino acid analogues were shown to increase the Ca/sup 2 +/ permeability of the plasma membrane of synaptosomes isolated from rat brain. Increased synaptosomal Ca/sup 2 +/ content was measured by uptake of /sup 45/Ca/sup 2 +/. Increased intrasynaptosomal free calcium was measured by the fluorescent calcium indicators indo 1 and quin 2. The increase in calcium permeability was maximal within 0.5 seconds (the limit of resolution of the methods used). The calcium permeability system(s) being modulated by the receptors appears to be Ca/sup 2 +/ channels but has properties different from the voltage dependent Ca/sup 2 +/ channels. Four different types of receptors have been observed; a kainate receptor antagonized by quisqualate, an L-cysteine sulfinate receptor, a 2-amino-2-phosphono-valerate receptor and an N-methyl-D-aspartate receptor. Each type of receptor is highly specific in ligand binding and none were activated or antagonized by aspartate or glutamate at concentrations up to 10 mM. Currently available data indicate substantial differences in ligand specificity between these presynaptic receptors and the postsynaptic receptors which have been classified by electrophysiological and radio-ligand binding studies. The neurotoxicity of kainate, L-cysteine sulfinate and N-methyl-D-aspartate may be due in part to metabolic and electrophysiological disturbances resulting from increased calcium in neurons having these receptors.
Research Organization:
Univ. of Pennsylvania Medical School, Philadelphia
OSTI ID:
5019728
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FLUORESCENCE
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGANDS
LUMINESCENCE
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
NERVES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES