Reductions in calcium uptake induced in rat brain synaptosomes by ionizing radiation

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577883· OSTI ID:6140389

Kandasamy, S B; Howerton, T C; Hunt, W A ^[1]

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1. Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)

Gamma irradiation (60Co) reduced KCl-stimulated voltage-dependent 45Ca2+ uptake in whole-brain, cortical, and striatal synaptosomes. The time course (3, 10, 30, and 60 s) of calcium uptake by irradiated (3 Gy) and nonirradiated synaptosomes, as well as the effect of KCl (15-65 mM), was measured in whole-brain synaptosomes. The fastest and highest rate of depolarization-dependent calcium uptake occurred at 3 s with 65 mM KCl. Irradiation reduced calcium uptake at all incubation times and KCl concentrations. Bay K 8644 enhancement of KCl-stimulated calcium influx was also reduced by radiation exposure. Nimodipine binding to dihydropyridine (DHP) L-type calcium channel receptors was not altered following radiation exposure. These results demonstrate an inhibitory effect of ionizing radiation on the voltage-sensitive calcium channels in rat brain synaptosomes that are not mediated by DHP receptors.

• OSTI ID: 6140389
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 125:2; ISSN RAREA; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CENTRAL NERVOUS SYSTEM
COBALT 60
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
EVEN-ODD NUCLEI
GAMMA RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
ODD-ODD NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOT