Comparison of the hypertrophic effect of phorbol ester, norepinephrine, angiotensin II and contraction on cultured cardiomyocytes
- Weis Center for Research, Danville, PA (United States)
Phorbol 12-myristate 13-acetate (PMA), norepinephrine (NE), angiotensin II (AII) and contraction stimulate cardiomyocyte growth. Differences exist in the time course and extent of protein and RNA accumulation. Cells plated at 4 {times} 10{sup 6} cells/60mm dish and arrested with 50 mM KCl demonstrated no significant growth. Treatment with PMA stimulated growth to a maximum of 17% at 48 h. In contrast, maximal stimulation of growth was 36% at 48 h and 31% at 72 h for contracting and NE treated cells, respectively. Maximal stimulation of the capacity for protein synthesis was 32% for PMA treated cells at 24 h as compared to 59% and 77% for NE treated and contracting cells respectively at 72 h. In support of a primary role for altered capacity in the regulation of protein synthesis, there was a significant correlation between RNA and protein content independent of the stimulus used. AII increased RNA content by 28% at 48h, but had no effect on growth up to 72h. Treatment with staurosporine blocked the stimulation of growth, suggestive of a role for protein kinase C (PKC). However, the inhibition of contraction-induced growth was due in part to a reduction in the rate of contraction. It was concluded that: significant differences existed in the time course of growth stimulation and RNA accumulation, depending on the stimulus; and growth inhibition by staurosporine is suggestive of an important role of PKC in hypertrophic growth induced by these stimuli.
- OSTI ID:
- 5239460
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of phorbol ester on 6-keto PGF sup 1. alpha. production in aorta from control-salt and aldosterone-salt hypertensive rats
Activation of glucose transport in skeletal muscle by phospholipase C and phorbol ester. Evaluation of the regulatory roles of protein kinase C and calcium
Related Subjects
ANGIOTENSIN
BIOSYNTHESIS
MUSCLES
CONTRACTION
NORADRENALINE
PHORBOL ESTERS
BIOLOGICAL EFFECTS
CELL CULTURES
COMPARATIVE EVALUATIONS
HEART
PHOSPHOTRANSFERASES
RNA
ADRENAL HORMONES
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DRUGS
ENZYMES
ESTERS
EVALUATION
GLOBULINS
HORMONES
NEUROREGULATORS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
SYMPATHOMIMETICS
SYNTHESIS
TRANSFERASES
VASOCONSTRICTORS
560300* - Chemicals Metabolism & Toxicology