Effect of phorbol ester on 6-keto PGF sup 1. alpha. production in aorta from control-salt and aldosterone-salt hypertensive rats

Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5264143

Jones, S B; Jones, A W ^[1]

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1. Univ. of Missouri, Columbia (United States)

The authors have previously shown that norepinephrine (NE) stimulated 6-keto-PGF{sub 1{alpha}} and thromboxane B{sub 2}(TXB{sub 2}) production in aorta from control-salt (CSR) and aldosterone-salt hypertensive rats (AHR) through the alpha-1 adrenoceptor (A1AR). While there was no difference in NE-stimulated TXB{sub 2} production between CSR and AHR, 6-keto-PGF{sub 1{alpha}} production was attenuated in aorta from AHR compared tissues. The authors were interested in whether the source of the arachidonic acid (AA) metabolites was through direct coupling of the A1AR and PLA{sub 2} or secondary to activation of PLC. One approach to answering this question was to bypass the receptor and activate protein kinase-C (PKC) directly. PMA caused a time-dependent increase in both 6-keto-PGF{sub 1{alpha}} and TXB{sub 2}. The time course was much slower than NE-stimulated production of these metabolites, but the pattern was similar with TXB{sub 2} appearing before 6-keto-PGF{sub 1{alpha}}. The PMA concentration-response curves for 6-keto-PGF{sub 1{alpha}} production for CSR and AHR were nearly superimposable. Staurosporine inhibited PMA stimulated 6-keto-PGF{sub 1{alpha}} production in CSR and AHR with nearly equal potency. Thus, while activation of PKC results in increases in AA metabolites, alterations in this pathway do not appear to be responsible for the differences observed with NE-stimulated 6-keto-PGF{sub 1{alpha}} production. These data support the concept of direct coupling between the A1AR and PLA{sub 2} in vascular smooth muscle.

• OSTI ID: 5264143
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AORTA
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARCINOGENS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ESTERS
HYPERTENSION
MAMMALS
MEMBRANE PROTEINS
METABOLITES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHORBOL ESTERS
PROSTAGLANDINS
PROTEINS
RATS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
SYMPTOMS
SYNTHESIS
TIME DEPENDENCE
VASCULAR DISEASES
VERTEBRATES