Combination therapy of a solid murine tumor with 1,3 bis (2-chloroethyl)-1-nitrosourea and irradiation

Conference · Sat Sep 01 04:00:00 EDT 1979 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:5237252

Begg, A C; Fu, K K; Vennari, J; Phillips, T L

The EMT6/SF tumor growing subcutaneously in mice was used to study the combined effects of BCNU and radiation, using both growth delay and in vitro assays. BCNU (15 mg/kg) appeared to potentiate the effect of x-rays (800 rad) when assayed by growth delay, the largest effect occurring when drug was given 1 to 8 hours after irradiation. The regrowth rates of tumors after combined treatment were slower than untreated tumors, but not slower than the x-ray control, and therefore any attempt to correct for the different regrowth rates did not alter the conclusion that potentiation between drug and radiation occurred. Of the 9 drugs studied in our laboratory, BCNU caused the largest amount of cell killing for a given growth delay when assayed in vitro. Consequently a lower drug dose of 7.5 mg/kg, giving a surviving fraction of 10%, was used with the in vitro assay. Cyclic variation in cell survival was seen as the interval between drug and radiation, and it varied between -48 and +48 hours. Maximum killing occurred when drug was given 1 hr after x-rays but was only slightly greater than expected from additivity. Tumor control (TCD/sub 50/) experiments on small tumors were also carried out to investigate the effects of high drug and radiation doses.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5237252
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:9
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL KILLING
DATA
DATA FORMS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
EXPERIMENTAL NEOPLASMS
GLANDS
GROWTH
IN VITRO
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
LOCAL IRRADIATION
MAMMALS
MAMMARY GLANDS
MICE
NEOPLASMS
NITROSO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
SURVIVAL CURVES
TIME DEPENDENCE
VERTEBRATES
X RADIATION