Response of the murine gastrointestinal epithelium to cis-dichlorodiammineplatinum II: radiation combinations

Conference · Wed Aug 01 04:00:00 EDT 1979 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:5236212

Burholt, D R; Schenken, L L; Kovacs, C J; Hagemann, R F

Single doses of cis-dichlorodiammineplatinum II (cis DDP) up to 8 mg/kg produced a dose-dependent inhibition of proliferative activity with a subsequent period of compensatory hyperplasia in the colon. The stomach was less responsive to cis DDP. Cis DDP-radiation combinations produced a dimunition in the proliferative response of the colon when compared to that following radiation only. This response was only seen in the stomach following 8 mg/kg of cis DDP and radiation. Cytokinetic analysis of the jejunal response to 8 mg/kg of cis DDP showed a gradual reduction in LN and MF/crypt with a reduction in the rate of DNA synthesis of S-phase cells through 8 hours post-treatment. By 24 hours the cellular DNA synthetic rate had recovered, although the number of S-phase cells was further reduced. The previously reported jejunal response to cis DDP-radiation combinations involved a reduction in crypt survival, coupled with a reduced proliferative capacity of surviving cells. Further investigation has revealed a possible inhibition of radiation damage repair by cis DDP, leading to increased levels of cell kill with the combination and reduced recovery of DNA synthetic rates in surviving cells.

• Research Organization: Allegheny General Hospital, Pittsburgh, PA
• OSTI ID: 5236212
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:8
• Country of Publication: United States
• Language: English

Related Subjects

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CELL PROLIFERATION
COMPARATIVE EVALUATIONS
COMPLEXES
CRYPT CELLS
DATA
DATA FORMS
DIGESTIVE SYSTEM
DNA
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
EXPERIMENTAL DATA
GASTROINTESTINAL TRACT
INFORMATION
INTESTINES
IRRADIATION
ISOLATED VALUES
LARGE INTESTINE
LOCAL IRRADIATION
MAMMALS
MICE
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PLATINUM COMPLEXES
RADIATION EFFECTS
RADIOSENSITIVITY EFFECTS
RODENTS
SMALL INTESTINE
SOMATIC CELLS
STOMACH
SYNTHESIS
TIME DEPENDENCE
TISSUES
TRANSITION ELEMENT COMPLEXES
VERTEBRATES