Changes in the composition of the extracellular fluid lining of the lung following biological, chemical, and physical insults

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5230917

Gurley, L R; London, J E; van der Kogel, A J; Dethloff, L A; Lehnert, B E

Measurements of the constituents in bronchoalveolar lavage fluids are of diagnostic value for lung disorders, including acute lung injury brought about by environmental insults and diseases resulting from exposure to pathogens and carcinogens. The authors have developed a high performance liquid chromatography (HPLC) method by which the proteins and other constituents in lung fluid can be fractionated. The ability of HPLC to identify and quantify pathological changes in lung fluid was demonstrated for three different cases. Rats testing positive for sendai virus and coronavirus were found to have both quantitative differences in protein components and a new disease-specific component. Rats subjected to thoracic X-irradiation were found to have both dose-dependent decreases and dose-independent increases in lung fluid constituents. Rats subjected to the intrapulmonary deposition of cadmium displayed large changes in lung fluid constituents derived from both the blood compartment and the lung's cellular compartment. These results demonstrate that HPLC can be used to provide a highly sensitive methods for detection of acute lung injury as well as other environmentally-induced alterations in the lung.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5230917
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BODY
BODY FLUIDS
CADMIUM
CHEMICAL COMPOSITION
CHROMATOGRAPHY
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELEMENTS
EXTRACELLULAR SPACE
IONIZING RADIATIONS
LAVAGE
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
MATERIALS
METALS
ORGANS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RADIATIONS
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SEPARATION PROCESSES
SPACE
TOXICITY
VERTEBRATES
X RADIATION