Analysis of proteins in bronchoalveolar lavage fluids during pulmonary edema resulting from nitrogen dioxide and cadmium exposure
We have developed a new HPLC method by which quantitative measurements can be made on the biochemical constituents of the extracellular fluid lining of the lung as sampled by bronchoalveolar lavage. Nine of the fractions are proteins, two are phospholipids, and two fractions remained unidentified. Rats were subjected to the intrapulmonary deposition of cadmium, a treatment model known to induce pulmonary edema and cause a translocation of blood compartment proteins into the lung's alveolar space compartment. Resulting pulmonary edema was hallmarked by /approximately/25-fold increases in three major blood compartment-derived HPLC protein fractions, two of which have been identified as albumin and immunoglobulin(s). Analysis of lavage fluid from rats exposed to 100 ppM NO/sub 2/ for 15 min, an exposure regimen which also produces pulmonary edema, indicated that the three blood compartment proteins in the lavage fluids were elevated 35- to 72-fold over controls 24 h after exposure. These results demonstrate that HPLC can be used to provide a highly sensitive method for detection and quantitation of pulmonary edema that can occur in acute lung injuries resulting from environmental insults.
- Research Organization:
- Los Alamos National Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6922770
- Report Number(s):
- LA-UR-88-2790; CONF-8808114-1; ON: DE88016159
- Country of Publication:
- United States
- Language:
- English
Similar Records
Development and characterization of an HPLC (high-performance liquid chromatography) system for the fractionation of bronchoalveolar lavage fluid
Changes in the composition of the extracellular fluid lining of the lung following biological, chemical, and physical insults
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Technical Report
·
Mon Aug 01 00:00:00 EDT 1988
·
OSTI ID:6908897
Changes in the composition of the extracellular fluid lining of the lung following biological, chemical, and physical insults
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5230917
Detection of metallothionein in bronchoalveolar cells and lavage fluid following repeated cadmium inhalation
Journal Article
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Fri Aug 01 00:00:00 EDT 1986
· Environ. Res.; (United States)
·
OSTI ID:5071160
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CADMIUM
CHALCOGENIDES
CHROMATOGRAPHY
EDEMA
ELEMENTS
KINETICS
LAVAGE
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
METALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SYMPTOMS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CADMIUM
CHALCOGENIDES
CHROMATOGRAPHY
EDEMA
ELEMENTS
KINETICS
LAVAGE
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
METALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SYMPTOMS
VERTEBRATES