Inhibition of protein synthesis in developing mouse brain after fission neutron irradiation in utero

Conference · Mon Jul 01 04:00:00 EDT 1985 · Radiat. Res.; (United States)

OSTI ID:5228170

Fonagy, A; Antal, S; Holland, J; Korosi, L; Hidvegi, E J

Previous investigations showed that when pregnant mice were exposed to a single whole-body dose of 0.5 Gy fission neutrons on Day 17 +/- 2 of gestation about 40% of the newborn mice died and the body and brain weights of surviving animals decreased by 30-35%. Studies presented in this paper show that after a single whole-body dose of 0.5 Gy neutron irradiation on Day 18 of pregnancy protein synthesis decreased in liver and brain of 3-week-old mice irradiated in utero. Incorporation of labeled amino acids in vivo into acid soluble nuclear proteins decreased by 15% in liver and by 40% in brain. Investigations with isolated protein synthesizing systems proved that the peptide bond formation was not impaired by irradiation. The aminoacylation of transfer-RNA, however, decreased in both liver and brain by 26-34 and 34-41%, respectively. Comparing the aminoacylation capacities in the two unirradiated organs, a much lower (about one-third) capacity was found in brain than in liver. Moreover, this low aminoacylation capacity of brain decreased further by about 40% after neutron irradiation. These results suggest that in the developing irradiated brain the reduced capacity of aminoacylation of transfer-RNA might be rate limiting for the efficiency of protein synthesis.

• Research Organization: Frederic Joliot-Curie National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary
• OSTI ID: 5228170
• Report Number(s): CONF-860423-
• Conference Information: Journal Name: Radiat. Res.; (United States) Journal Volume: 103:1
• Country of Publication: United States
• Language: English

Related Subjects

560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
BRAIN
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
ELEMENTARY PARTICLES
EXTERNAL IRRADIATION
FERMIONS
FISSION NEUTRONS
GLANDS
HADRONS
HISTONES
INHIBITION
IRRADIATION
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
MICE
NERVOUS SYSTEM
NEUTRONS
NUCLEIC ACIDS
NUCLEONS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PRENATAL EXPOSURE
PRENATAL IRRADIATION
PROTEINS
RADIATION EFFECTS
RNA
RODENTS
SYNTHESIS
TRANSFER RNA
VERTEBRATES
WHOLE-BODY IRRADIATION