Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism
- Thoraxklinik Heidelberg-Rohrbach, Heidelberg, (West Germany)
Exposure of cultures of human lung fibroblasts to 0.5 ppm ozone for 20 h resulted in a significant increase in cellular mortality by 29%; after exposure to 2.5 ppm ozone for 4 h, the increase amounted to 74%. A marked difference in sensitivity to ozone was observed between fibroblast lines from different individuals. This variability in resistance to ozone was more evident after exposure to 0.5 ppm ozone for 20 h, when compared with 2.5 ppm ozone for 4 h. In one fibroblast line, synthesis of hyaluronan was enhanced by exposure to 0.5 ppm ozone for 20 h. The concentrations of hyaluronan in culture media increased in experiments using different fibroblast cell lines, a phenomenon that was obvious both if cell numbers and combined protein concentrations of cells and media are selected as references for hyaluronan concentrations.
- OSTI ID:
- 5227045
- Journal Information:
- Journal of Toxicology and Environmental Health; (United States), Vol. 35:4; ISSN 0098-4108
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
CELL KILLING
HYALURONIC ACID
METABOLISM
OZONE
TOXICITY
CELL CULTURES
CULTURE MEDIA
LUNGS
MAN
PROTEINS
AMINES
ANIMAL CELLS
ANIMALS
BODY
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
MAMMALS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PRIMATES
RESPIRATORY SYSTEM
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology