Analysis of the diversity of murine antibodies to dextran B1355. II. Demonstration of multiple idiotypes with variable expression in several strains. [Radioimmunoassay, /sup 125/I tracer technique]

Journal Article · Sat Oct 01 04:00:00 EDT 1977 · J. Immunol.; (United States)

OSTI ID:5214283

Hansburg, D; Briles, D E; Davie, J M

We have developed radioimmunoassays that detect idiotypic (variable region) differences among the ..cap alpha..(1 ..-->.. 3) dextran-binding myeloma proteins U102, J558, and M104 as well as an assay that detects variable region determinants common to all three proteins. Using these assays, we have examined 7S and 19S anti-..cap alpha..(1 ..-->.. 3) dextran antibodies induced in five murine strains of the a/sup 1/I/sub g/C/sub H/ linkage group and the recombinant strain BAB/14. All idiotypes were expressed in both 19S and 7S antibodies from all strains, but with considerable strain-specific variability in penetrance. In two strains, one additional type of antibody, which lacked all four idiotypic determinants, generally constituted the bulk of total anti-..cap alpha..(1 ..-->.. 3) dextran antibodies.

• Research Organization: Washington Univ. School of Medicine, St. Louis
• OSTI ID: 5214283
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 119:4; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BLOOD SUBSTITUTES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DEXTRAN
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES