Special proliferative sites are not needed for seeding and proliferation of transfused bone marrow cells in normal syngeneic mice

Journal Article · Sun Aug 01 04:00:00 EDT 1982 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.79.16.5085· OSTI ID:5213830

Brecher, G ^[1]; Ansell, J D; Micklem, H S; Tjio, J H; Cronkite, E P

---

1. Univ. of California, Berkeley

The widely held view that transfused bone marrow cells will not proliferate in normal mice, not exposed to irradiation or other forms of bone marrow ablation, was reinvestigated. Forty million bone marrow cells from male donors were given to female recipients on each of 5 consecutive days, 5 to 10 times the number customarily used in the past. When the recipients were examined 2-13 weeks after the last transfusion, donor cells were found to average 16-25% of total marrow cells. Similar percentages of donor cells were found when variants of the enzyme phosphoglycerate kinase determined electrophoretically were used for identification of donor and recipient cells. Evidence is presented that the proportion of donor cells is compatible with a nonlinear dependence on the number of cells transfused over the range tested - i.e., 20-200 million bone marrow cells injected intravenously. Special proliferative sites thus do not appear to be required.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5213830
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 79; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BODY
BONE MARROW CELLS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL PROLIFERATION
CFU
CONNECTIVE TISSUE CELLS
GLUCOSE
HEXOSES
IRRADIATION
LABELLED COMPOUNDS
LETHAL IRRADIATION
MAMMALS
MICE
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN
THERAPY
TRANSFUSIONS
VERTEBRATES