The effect of dietary pyridoxine on arsenic deprivation in rats
- Dept. of Agriculture, Grand Forks, ND (United States)
In experiments on As deprivation, many findings indicate that As can affect enzymes or metabolites that are also influenced by vitamin B{sub 6}. Thus, an experiment was designed to ascertain the effect of pyridoxine (pyr) on As deprivation in rats. Male, weanling rats were fed an amino acid based diet containing 0.24% methionine (M) and less than 15 ng As/g. Dietary variables were As, 0 or 1 {mu}g/g; M, 0 or 3 g/kg; and pyridoxine, 0 or 10 mg/kg. After 10 weeks, growth was reduced by As, Pyr, or M deprivation. Both endogenous ({minus}PP) and pyridoxal phosphate-stimulated (+PP) RBC aspartate aminotransferase were decreased by Pyr deficiency. The ratio of +PP/{minus}PP, known as the activation coefficient (AC), was affected by an interaction between As and Pyr. Pyr deficiency resulted in a less marked increase in AC in the As-deprived rats than in the As-supplemented rats. Plasma Fe was slightly decreased by Pyr deficiency in the As-deprived rats but increased by Pyr deficiency in the As-supplemented rats. Plasma threonine and serine were increased by As supplementation in the Pyr-deficient rats but there was no effect of As supplementation in the Pyr-supplemented rats. Plasma alanine was decreased by As or Pyr deprivation. In Pyr deficiency, As deprivation had no effect on plasma glycine (G) in the M-deficient rats but decreased G in the M-supplemented rats. In the Pyr-supplemented rats, As had no effect on G, regardless of M. The findings indicate that As and Pyr interact to affect amino acid metabolism.
- OSTI ID:
- 5213156
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AMINOTRANSFERASES
ANIMALS
ARSENIC
AZINES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
DIET
ELEMENTS
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
METABOLISM
NITROGEN TRANSFERASES
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIDINES
PYRIDOXINE
RATS
RODENTS
SEMIMETALS
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS