Activation of transforming potential of the human insulin receptor gene

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.16.5725· OSTI ID:5209520

Wang, L H; Lin, B; Jong, S M.J.; Dixon, D; Ellis, L; Roth, R A; Rutter, W J

A retrovirus containing part of the human insulin receptor (hIR) gene was constructed by replacing ros sequences in the avian sarcoma virus UR2 with hIR cDNA sequences coding for 46 amino acids of the extracellular domain and the entire transmembrane and cytoplasmic domains of the ..beta.. subunit of hIR. The resulting virus, named UIR, contains the hIR sequence fused to the 5' portion of the UR2 gag gene coding for p19. UIR is capable of transforming chicken embryo fibroblasts and promoting formation of colonies in soft agar; however, it does not form tumors in vivo. A variant that arose from the parental UIR is capable of efficiently inducing sarcomas in vivo. UIR-transformed cells exhibit higher rates of glucose uptake and growth than normal cells. The 4-kilobase UIR genome codes for a membrane-associated, glycosylated gag-hIR fusion protein of 75 kDa designated P75/sup gag-hir/. P75/sup gag-hir/ contains a protein tyrosine kinase activity that is capable of undergoing autophosphorylation and of phosphorylating foreign substrates in vitro; it is phosphorylated at both serine and tyrosine residues in vivo

• Research Organization: Rockefeller Univ., New York, NY (USA)
• OSTI ID: 5209520
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:16
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ONCOGENIC TRANSFORMATIONS
GENE REGULATION
SERINE
PHOSPHORYLATION
TYROSINE
ENZYME ACTIVITY
FIBROBLASTS
GENES
LEUCINE
ONCOGENIC VIRUSES
PHOSPHORUS 32
PHOSPHOTRANSFERASES
RECEPTORS
RECOMBINANT DNA
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
HYDROXY ACIDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
TRANSFERASES
VIRUSES
550201* - Biochemistry- Tracer Techniques