Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Heterologous transmembrane signaling by a human insulin receptor-v-ros hybrid in Chinese hamster ovary cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ; ;

A hybrid receptor molecule composed of the extracellular ligand-binding domain of the human insulin receptor and the transmembrane and cytoplasmic (protein-tyrosine kinase) domains of the chicken sarcoma virus UR2 transforming protein p68/sup gag-ros/ has been constructed and expressed in Chinese hamster ovary (CHO) cells. The hybrid is processed normally into ..cap alpha.. and hybrid ..beta.. subunits, is expressed on the cell surface at high levels, and binds insulin with near-wild-type affinity. Furthermore, insulin stimulates the phosphorylation on tyrosine resides of the hybrid ..beta..-subunit in vivo and the phosphorylation of an exogeneous substrate (poly(Glu,Tyr)) in vitro. Thus the hybrid is capable of heterologous transmembrane signaling. However, the hybrid mediates neither the insulin-activated uptake of 2-deoxyglucose nor the incorporation of (/sup 3/H)thymidine into DNA, suggesting that the physiological response(s) mediated by ligand-activated protein-tyrosine kinases may utilize distinct intracellular mechanisms for postreceptor signaling

Research Organization:
Univ. of California, San Francisco (USA)
OSTI ID:
5209820
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:15; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Activation of transforming potential of the human insulin receptor gene
Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5209520
Identification of the insulin receptor tyrosine residues undergoing insulin-stimulated phosphorylation in intact rat hepatoma cells
Journal Article · Mon Jan 04 23:00:00 EST 1988 · J. Biol. Chem.; (United States) · OSTI ID:6837967
Nature and regulation of the insulin receptor: structure and function
Journal Article · Mon Dec 31 23:00:00 EST 1984 · Annu. Rev. Physiol.; (United States) · OSTI ID:5150237

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHO CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METHIONINE
MOLECULAR STRUCTURE
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RECEPTORS
RIBOSIDES
SULFUR 35
SULFUR ISOTOPES
THYMIDINE
TRANSFERASES
TRITIUM COMPOUNDS