Methods for estimating the contribution of proenkephalin A and proenkephalin B input to neuronal areas

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(86)24043-4· OSTI ID:5205663

Sundberg, D K; Dunlap, III, C E

Final expression of the proenkephalin A and proenkephalin B (prodynorphin) gene should yield a fixed ratio of enkephalin-like peptides depending on their precursor origin. If a tissue contained peptides only from proenkephalin A this ratio would be 4/1/1/1 (Met-Enk/Leu-Enk/Met-Enk-Arg6-Phe7/Met-Enk-Arg6-Gly7-Leu8). On the other hand, if the tissue-derived opiates were from prodynorphin, only Leu-enkephalin should be found. The techniques described here combining oxidation of the Met-Enk species, reverse-phase HPLC, and measurement with a radioimmunoassay which recognized many of the opiate-like peptides have been found useful for determining the precursor origin of enkephalins within different biological sources.

• OSTI ID: 5205663
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States); ISSN MENZA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
DISTRIBUTION
DRUGS
ENDORPHINS
ENKEPHALINS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
NERVE CELLS
NEUROREGULATORS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SOMATIC CELLS
TISSUE DISTRIBUTION
TRACER TECHNIQUES